Dengue IgG/IgM Tri-line Rapid Test Cassette

Dengue IgG/IgM Tri-line Rapid Test Cassette is an in vitro diagnostic tool used detect both IgG and IgM antibodies to the dengue virus simultaneously. It employs the colloidal gold

immunochromatography technique, which is characterized by its rapid simple, and accurate performance, making it suitable for early diagnosis and infection staging of dengue fever. I. Principle of Detection Colloidal Gold Immunromatography: This test card uses dengue recombinant antigen labeled with colloidal gold to bind with dengue IgG or IgM antibodies in the, forming a complex. During the

chromatography process, the complex binds with the anti-human IgG or IgM antibodies fixed on the nitrocellulose membrane forming a sandwich that condenses and colors. Tri-line Design: The test card is usually designed with three detection lines, corresponding to IgG antibody, IgM antibody, the control line. The control line is used to verify the effectiveness of the

detection process. II. Significance of Detection IgM Antibody Detection:M antibodies usually appear within 5-7 days after infection and last for several weeks, indicating acute infection. A positive IgM antibody result may suggest that the

patient is in the stage of dengue fever infection. IgG Antibody Detection: IgG antibodies usually appear within 2 weeks after infection and last for a long time, indicating infection or immune protection. A positive IgG antibody result may indicate

that the patient has been infected with the dengue virus or has developed immune protection. Advantages of Testing: By detecting both IgG and IgM antibodies simultaneously, it is possible to more accurately determine the patient's infection status, including the infection stage

(acute, conescent, or past infection) and immune status. III. Technical Features Rapid: The detection time is usually within 15-30 minutes, meeting clinical need for rapid diagnosis. Simple: Easy to operate without the need for professional laboratory equipment, suitable for various medical institutions and sites. Accurate: It has high accuracy specificity and can effectively distinguish between different types of dengue virus infections. Convenient: The sample types are diverse, including whole blood, serum, or plasma, which convenient for collection and testing. IV. Precautions for Use Sample Collection: Samples should be collected strictly according to the instructions to avoid contamination or confusion. Det Operation: The operation should be carried out strictly according to the instructions, including sample dilution, addition, and result reading steps. Result Interpretation: Results should be read the specified time to avoid false positives or false negatives caused by reading after the time limit. Control Line: The control line must be colored; otherwise, the detection result is invalid Storage Conditions: The test card should be stored in a dry, dark, and cool place, avoiding high temperatures, humidity, or freezing